PhD in Biotechnology Engineering (Tufts University) with emphasis in synthetic biology. Bioprocess engineer at bachelor’s and master’s level (Federal University of Paraná). Experience in synthetic biology, industrial microbiology and molecular biology.
GPA: 0.9029 (out of 1.0000)
Cloning and expression of recombinant enzymes in yeasts (S. cerevisiae, K. lactis, P. pastoris). Production of enzymes for animal feed and biofuel production in small scale bioreactors (5, 15, 50 L).
Bench scale alcoholic fermentation by S. cerevisiae and analysis of DNA fingerprint of yeasts isolated in bioethanol plants.
Molecular biology methods for knockouts in Trypanosoma cruzi flagelar gene.
Submerged fermentation of lactic bacteria and edible mushrooms in shake flasks and bench-top bioreactors (2 L). Extraction and analysis of polysaccharides. In vivo (mice) and in vitro antitumoral and antioxidant activity assays.
NASCIMENTO, B. M.; NAIR, N. U. Characterization of a membrane enzymatic complex for heterologous production of poly-γ-glutamate in E. coli. Metabolic Engineering Communications, v. 11, e00144, 2020. doi: 10.1016/j.mec.2020.e00144
LIMA, L. F. O.; HABU, S.; GERN, J. C.; NASCIMENTO, B. M.; PARADA, J.-L.; NOSEDA, M. D.; GONÇALVES, A. G.; NISHA, V. R.; PANDEY, A.; SOCCOL, V. T.; SOCCOL, C. R. Production and characterization of the exopolysaccharides produced by Agaricus brasiliensis in submerged fermentation. Applied Biochemistry and Biotechnology, v. 151, p.283-294, 2008. doi: 10.1007/s12010-008-8187-2
